2015 Fiscal Year Final Research Report
The roles of downstream factor(s) of cancer cell-derived Klotho on bone mineralization
| Project/Area Number |
25462858
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Morphological basic dentistry
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| Research Institution | Hiroshima University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
YOSHIKO YUJI 広島大学, 大学院医歯薬保健学研究院, 教授 (20263709)
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| Co-Investigator(Renkei-kenkyūsha) |
FUTAMURA MANABU 岐阜大学, 医学部, 准教授 (10415515)
KOZAI KATSUYUKI 広島大学, 大学院医歯薬保健学研究院, 教授 (10178212)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Keywords | Klotho / FGF23 / 骨代謝 / リン代謝 / 骨転移 |
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| Outline of Final Research Achievements |
Soluble Klotho (sKL) was detected in the several cancer cell lines and stimulated ERK signaling in vitro in the combination with FGF23 which is derived from bone. However, sKL-mediated ERK signaling was tissue-specifically regulated because of the presence of the protease(s) by which FGF23 was processed.
Downstream factor of sKL and FGF23, X, was identified by DNA microarray. X inhibited bone matrix mineralization in vivo and in vitro, but did not affect on proliferation and differentiation in both osteoblast and osteoclast cell cultures. X was strongly detected in bone matrix. osteoblasts and osteocytes with immunohistochemistry by anti-X polyclonal antibody.
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| Free Research Field |
形態系基礎歯科学
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