Establishment of a Cre-conditional Plag1-driven salivary gland tumor mouse model.

2015 Fiscal Year Final Research Report

• Project/Area Number: 25462875
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Morphological basic dentistry
• Research Institution: Showa University
• Principal Investigator: Irie Tarou 昭和大学, 歯学部, 講師 (00317570)
• Co-Investigator(Kenkyū-buntansha): MISHIMA Kenji 昭和大学, 歯学部, 教授 (50275343) ; MORI Taisuke 国立研究開発法人国立がん研究センター, 分子病理分野, 研究員 (00296708) ; YASUHARA Rika 昭和大学, 歯学部, 助教 (20453649) ; YAMAMOTO Go 昭和大学, 歯学部, 兼任講師 (80384189)
• Co-Investigator(Renkei-kenkyūsha): TSUJI Takashi 国立研究開発法人理化学研究所, 多細胞システム形成研究センター, チームリーダー (50339131) ; TOYOSHIMA Koh-ei 北里大学, 医学部, 特任講師 (00599243)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 唾液腺腫瘍 / モデルマウス / 腫瘍組織発生 / コンディショナルマウス

Outline of Final Research Achievements

Details of histogenesis of salivary gland tumors are still not entirely clear. We performed challenge for establishment of a Cre-conditional Plag1-driven salivary gland tumor mouse model which causes PLAG1 overexpression only in basal, myoepithelial or luminal cells of salivary gland. Two independent lines of Plag1 overexpressed (CAG-loxp-lacZ-pA-loxp-Plag1-EGFP) flox mouse were established. Two kinds of conditional mouse which overexpressed PLAG1 only in luminal cells (Sox9-CreERT2-loxP mouse) or myoepithelial cells (Myh11-Cre-loxP mouse) of salivary glands are available at the present time. Moreover, we established three stable PLAG1 overexpressed cell lines of acinar-, duct- and myoepithelial phenotype of human normal salivary glands. Our results by use of these stable cell lines indicated that PLAG1 may play a dual role in salivary gland tumorigenesis in cell specific manner.

Free Research Field

口腔病理学