Pathological analysis of autoimmune sialadenitis using human dental pulp

2015 Fiscal Year Final Research Report

• Project/Area Number: 25462878
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Morphological basic dentistry
• Research Institution: Tokyo Dental College
• Principal Investigator: Muramatsu Takashi 東京歯科大学, 歯学部, 教授 (00276982)
• Co-Investigator(Kenkyū-buntansha): Ryo Koufuchi 鶴見大学, 歯学部, 講師 (10298268) ; Mishima Kenji 昭和大学, 歯学部, 教授 (50275343) ; Inoue Hiroko 日本薬科大学, 薬学部, 准教授 (50367306) ; Ichiro Sito 鶴見大学, 歯学部, 教授 (60147634)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 唾液腺 / シェーグレン症候群 / 歯髄細胞 / 分化転換

Outline of Final Research Achievements

To investigate mechanism of autoimmune sialoadenitis, transdifferentiation from pulp cells to salivary gland cell was tested using normal and Sjogren syndrome human dental pulp tissues. Co-culture of normal pulp cells and salivary gland cell was carried out and expression of salivary gland cell was investigated using RT-PCR. Dental pulp cell expressed aquaporin-5 (AQP-5) after co-culture of salivary gland cells. However, other markers such as amylase were not detected. The result suggests that transdifferentiation from dental pulp to salivary gland cells is difficult using co-culture technique.

Free Research Field

歯科保存学