2015 Fiscal Year Final Research Report
Screening of novel inhibitors to hydrogen sulfide-producing enzymes -based on enzyme structures and reaction mechanisms- that can prevent oral malodor
| Project/Area Number |
25462897
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Functional basic dentistry
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| Research Institution | Iwate Medical University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
YOSHIDA Yasuo 愛知学院大学, 歯学部, 准教授 (10315096)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Keywords | 硫化水素産生酵素 / 酵素 / 歯周病 / 口臭 / 化合物スクリーニング |
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| Outline of Final Research Achievements |
Structral and enzymatic analyses of hydrogen sulfide-producing enzymes from a periodontal pathogen, Fusobacterium nucleatum, were performed. We identified four important amino acid residues on catalysis and/or substrate (reaction intermediate) binding, and revealed their roles and contributions to the catalytic activity. On the basis of these results, the reaction mechanism of hydrogen sulfide-producing enzyme Fn1055 was proposed. In parallel, we constructed two assay systems for inhibitor screening. Using the assay systems, small molecules from chemical libraries were screened.
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| Free Research Field |
構造生物学
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