Effect of lipid mediators on osteoclast differentiation

2016 Fiscal Year Final Research Report

• Project/Area Number: 25462899
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Functional basic dentistry
• Research Institution: Osaka Dental University (2014-2016); Showa University (2013)
• Principal Investigator: AMANO Hitoshi 大阪歯科大学, 歯学部, 准教授 (90212571)
• Co-Investigator(Renkei-kenkyūsha): ISHII Masaru 大阪大学, 大学院医学系研究科, 教授 (10324758)
• Project Period (FY): 2013-04-01 – 2017-03-31
• Keywords: 活性脂質 / S1P / 破骨細胞 / 分化

Outline of Final Research Achievements

Sphingosine-1-phosphate (S1P), which is mainly erythrocyte-derived, is a lipid mediator, which exists at high levels in plasma and acts on its receptors. S1P displays physiological functions through the S1P receptor. This study examined the influence of S1P in terms of controlling osteoclastic differentiation. Upon separation of erythrocytes from all bone marrow cells, the level of osteoclast formation decreased; subsequently, following the addition of S1P, the number of cells recovered. Furthermore, the number of osteoclasts decreased in the S1P1/3 antagonist. RT-PCR revealed that S1P1 mRNA expression is higher in bone marrow cells in comparison to osteoclasts; in contrast, S1P2 mRNA expression is higher in osteoclastic cells. On the other hand, S1P1 function was aided and cell differentiation was accelerated by inhibition of S1P2. Based on the aforementioned findings, it is clear that S1P plays an important role in the regulation of osteoclast differentiation.

Free Research Field

歯科薬理学