2015 Fiscal Year Final Research Report
Basic research for development of osteoregeneration therapy using mesenchymal stem cells and iPS cells
| Project/Area Number |
25462900
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Functional basic dentistry
|
|---|
| Research Institution | Tokyo Dental College |
|---|
Principal Investigator |
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
SHINO Hiromi 東京歯科大学, 歯学部, 助教 (00445446)
ONODERA Shoko 東京歯科大学, 歯学部, 助教 (90637662)
|
|---|
| Project Period (FY) |
2013-04-01 – 2016-03-31
|
| Keywords | iPS細胞 / 骨代謝 / 骨芽細胞 / 再生医療 / 疾患特異的iPS細胞 / 骨細胞 / Wnt / ヘッジホッグ |
|---|
| Outline of Final Research Achievements |
We developed new strategies to generate all stages of osteogenic cells including mesenchymal stem cells, pre-osteoblasts, osteoblast and osteocytes. These cells are expressed their specific marker proteins. We found that PI3K-Akt signaling is essential for early stage of osteogenesis. IGF-1, which is an effective stimulator of PI3K-Akt signaling is a potent inducer of osteogenesis. We found that IGF-1 treatment significantly improve osteogenesis. We established an effective protocol for generating osteocyte from iPS cells. We utilized this protocol to develop new strategy for osteo-regeneration therapy. We transplanted osteoblasts derived from human iPS cells to bone defects generated in Rat and found successful regeneration.We found that ALP is a specific and efficient surface marker to isolate osteolineage cells derved from iPS cells. We established effective protocol to generate osteoblast from human patient derived iPS cells.
|
| Free Research Field |
生化学
|
