2015 Fiscal Year Final Research Report
Functional Analysis of Apoptosis Regulatory Factor GRIM19 in Oral Carcinoma Cells
| Project/Area Number |
25462929
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Pathobiological dentistry/Dental radiology
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| Research Institution | Meikai University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
Yoshihiro Ohmori 明海大学, 歯学部, 教授 (50194311)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Keywords | GRIM19 / 扁平上皮癌 / アポトーシス / ＩＦＮ / オートファジー / STAT3 |
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| Outline of Final Research Achievements |
We have investigated the expression of the GRIM-19 in oral squamous cell carcinoma cell lines and found a low-GRIM-19 expression cell line (HSC-2) and a high- GRIM-19 expression cell line (Ca9-22). Our results also suggested that the expression of the GRIM-19 is regulated at the protein levels but not transcriptional level. To determine the regulatory mechanism for the expression of GRIM-19 protein, we analyzed the involvement of ubiquitin-proteasome systems and autophagy system using various inhibitors. The results demonstrated that the expression of GRIM-19 protein unregulated by treatment with the proteasome and the autophagy inhibitors in Ca9-22 cells. However neither the proteasome inhibitor nor the autophagy inhibitor enhanced the expression in HSC-2 cells. These results suggest a possibility that the impaired expression of GRIM-19 in HSC-2 cells is due to a mutation in the cording region of the GRIM-19 gene.
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| Free Research Field |
口腔外科学
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