Proliferating activity of cryopreserved periodontal ligament cells

2015 Fiscal Year Final Research Report

• Project/Area Number: 25463073
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Surgical dentistry
• Research Institution: Niigata University
• Principal Investigator: ONO Yukiko 新潟大学, 医歯(薬)学総合研究科, 非常勤講師 (80345511)
• Co-Investigator(Kenkyū-buntansha): YOSHIZAWA Michiko 新潟大学, 医歯学総合病院, 講師 (60303137)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 凍結保存 / 歯 / 歯根膜 / 幹細胞 / 培養 / 細胞工学 / 再生医療

Outline of Final Research Achievements

The purpose of this study was to compare the effect of some cryopreservation methods on periodontal regeneration of rat teeth in vitro. The maxillary molars of 4-week old Wister rats were extracted and frozen by gradual freezing method using a programmed freezer or super cooling-rapid freezing method for 1, 3, 6, 9, 12 months, and then thawed in a water bath at 37°C. The periodontal ligament cells were isolated and cultured. Cell proliferation of super cooling-rapid freezing group was slightly higher than gradual freezing one, but there was no significant difference.

Free Research Field

口腔外科学