Immunohistochemical and cell biological studies on the epithelial lining in periapical lesions - expression of epithelial mesenchymal transition (EMT) markers and antimicrobial peptides -

2015 Fiscal Year Final Research Report

• Project/Area Number: 25463094
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Surgical dentistry
• Research Institution: Kagoshima University
• Principal Investigator: Ishihata Kiyohide 鹿児島大学, 医歯学域医学部・歯学部附属病院, 助教 (10437957)
• Co-Investigator(Kenkyū-buntansha): NAKAMURA NORIFUMI 鹿児島大学, 医歯学域歯学系, 教授 (60217875) ; KOMATSUZAWA HITOSHI 鹿児島大学, 医歯学域歯学系, 教授 (90253088) ; SHIMA KAORI 鹿児島大学, 医歯学域歯学系, 助教 (10343526)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 歯原性上皮細胞 / 細菌感染 / 歯根嚢胞 / EMT / 口腔外科

Outline of Final Research Achievements

Based on the hypothesis that periapical lesions may play an important role as a second barrier in defense mechanisms against bacterial infections, we examined the development of the epithelial lining in periapical lesions and the expression of antimicrobial peptides and EMT markers in tissue specimens. We also investigated the relationship between EMT markers and bacterial infection using immortalized cells. In an attempt to reduce lymphocyte in apical granulomas, an epithelial barrier develops on the surface of the cavity and expresses E-cadherin and β-defencine. Bacterial infection accelerates the expression of E-cadherin in vivo and in vitro; however, the intervention of N-cadherin was not detected in the present study.

Free Research Field

医歯薬学