2015 Fiscal Year Final Research Report
Development of new treatment strategy targeting for vascular niche of oral cancer stem cell
Project/Area Number |
25463106
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Surgical dentistry
|
Research Institution | Hokkaido University |
Principal Investigator |
OHIRO Yoichi 北海道大学, 歯学研究科(研究院), 講師 (40301915)
|
Co-Investigator(Kenkyū-buntansha) |
AKIYAMA Kousuke 北海道大学, 遺伝子制御研究所, 客員研究員 (10609100)
TEI Kanchu 北海道大学, 歯学研究科(研究院), 教授 (80180066)
|
Project Period (FY) |
2013-04-01 – 2016-03-31
|
Keywords | 腫瘍血管内皮細胞 / がん幹細胞 / 薬剤抵抗性 |
Outline of Final Research Achievements |
Cancer stem cells (CSC) or matured cancer cells were fractionated from cancer cell lines. Tumor endothelial cells (TEC) were separated from transplanted tumors in vivo model. CSC were co-cultured with TEC or treated with TEC conditioned media (TEC-TM). These experiments revealed that activation of survival signal such as Akt or NF-kB, alteration of expression levels of CSC markers and inhibitory effect against apopotosis were induced in CSC. Furthermore, co-cultivation of CSC with TEC separated from highly metastatic tumor induce the formation of large sphenoid or inhibitory effect for anoikis by TEC.
|
Free Research Field |
外科系歯学
|