Preliminary study of siRNA transfection into embryonic lingual tissue to establish the technique for lingual papillae regeneration

2015 Fiscal Year Final Research Report

• Project/Area Number: 25463153
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Surgical dentistry
• Research Institution: The Nippon Dental University
• Principal Investigator: Yoshimura Ken 日本歯科大学, 新潟生命歯学部, 准教授 (90297953)
• Co-Investigator(Renkei-kenkyūsha): NASHIDA TOMOKO 日本歯科大学, 新潟生命歯学部, 准教授 (10133464) ; MIKAMI MASATO 日本歯科大学, 新潟生命歯学部, 講師 (90173997)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 遺伝子導入 / siRNA / 舌乳頭形態形成

Outline of Final Research Achievements

Essential proteins for lingual papillae morphogenesis are likely present during embryonic development. We searched for the responsible genes and selected candidates involved in lingual papillae morphogenesis. Then to establish regeneration techniques, preliminary transfection of siRNA was performed. Transfection of the synthesized siRNA together with vectors for fluorescent proteins expression into rat embryonic lingual tissues was performed using the electroporation method. After the transfection, the lingual tissues were organ-cultured and observed. Twenty-four hours after the transfection, fluorescence markers started illuminating inside of the lingual tissues and were continuing to glow up to the 72 hour intervals. However, the quality of fluorescence was being occasionally unstable. Optimization of electric pulse parameters was required in order to maintain cell viability and more efficacious transfection methods also would be required.

Free Research Field

口腔解剖学