2016 Fiscal Year Final Research Report
Elucidation of epigenetic mechanism in gingival epithelium by periodontal pathogen
| Project/Area Number |
25463223
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Periodontology
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| Research Institution | Health Sciences University of Hokkaido |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2017-03-31
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| Keywords | エピジェネティクス / 歯周疾患 / 線維芽細胞 / DNAメチル化 / LPS / 歯周病原菌 / 歯周組織 |
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| Outline of Final Research Achievements |
In this study, we investigated epigenetics change when P. gingivalis-derived LPS was allowed to act on human gingival fibroblasts for a long time. LPS chronic stimulation was given to human gingival fibroblasts, DNA hypermethylation and mRNA expression decrease of fibronectin type III (FN), αI (XII) collagen (XII-collagen) and extracellular matrix protein related genes were observed . Protein analysis also reduced the expression of XII-collagen and FN and its expression was restored by demethylating agent.These result suggest that periodontal pathogen-derived LPS is involved in the pathogenesis and progression of periodontal disease due to gene expression reduction through epigenetic modification.
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| Free Research Field |
臨床口腔病理
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