2015 Fiscal Year Final Research Report
Cell culture conditions regulates histone modification.
| Project/Area Number |
25503002
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
epigenetics
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| Research Institution | Tokyo Institute of Technology (2014-2015)
Osaka University (2013) |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Keywords | ヒストン修飾 / 細胞培養 / メチル化 / アセチル化 |
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| Outline of Final Research Achievements |
The levels of histone modifications change depending on the surrounding environments. To validate the effects of cell culture condition on histone modification, cells were cultured under various cell conditions and the levels of histone modifications were measured.
When dense cell cultured supernatant were added to the culture medium, the levels of histone acetylation, and methylation, increased and decreased, respectively. The levels of histone acetylation changed within 3 hr, which was relatively fast. On the other hands, the levels of methylation decreased after 24 hr, suggesting that the histone acetylation and methylation are regulated by different mechanism. Furthermore, we found that one of the factor which changed histone modifications in dense cell cultured supernatant was lactic acid.
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| Free Research Field |
細胞生物学
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