Analysis of differentiation control gene in mesenchymal stromal cells

2015 Fiscal Year Final Research Report

• Project/Area Number: 25505001
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Regenerative medicine
• Research Institution: Tokyo Medical and Dental University
• Principal Investigator: AKAZAWA Chihiro 東京医科歯科大学, 大学院保健衛生学研究科, 教授 (80291160)
• Co-Investigator(Kenkyū-buntansha): SUZUKI Nobuharu 東京医科歯科大学, 大学院保健衛生学研究科, 准教授 (30596565) ; MABUCHI Yo 東京医科歯科大学, 大学院保健衛生学研究科, 助教 (50424172)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 間葉系幹細胞 / 遺伝子制御

Outline of Final Research Achievements

Mesenchymal stromal cells (MSCs) have the differentiation ability to mesenchymal lineage cells in culture and maintain the quiescent state in adult tissues. Because MSCs have been defined using cells cultured in vitro, discrepancies have arisen between studies concerning their properties. Using prospective isolation method for human and mouse MSCs, we performed the clonal analysis of MSCs about maintaining the quiescence. Clonal characterization of MSCs demonstrated that the transmembrane protein teneurin-4 (Ten-4) is highly expressed in the quiescent MSCs. The cell culture analysis showed that Ten-4 was downregulated in MSC after differentiation. Our findings revealed that Ten-4 player a role in maintaining the quiescence of MSCs.

Free Research Field

再生医学