Research for treatment of refractory arrhythmia with induced-pluripotent stem cells-derived cardiomyocytes

2015 Fiscal Year Final Research Report

• Project/Area Number: 25505004
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Regenerative medicine
• Research Institution: Keio University
• Principal Investigator: Jun Fujita 慶應義塾大学, 医学部, 講師 (10306706)
• Co-Investigator(Renkei-kenkyūsha): FUKUDA Keiichi 慶應義塾大学, 医学部, 教授 (20199227)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 再生医療 / iPS細胞 / 再生心筋細胞 / 不整脈治療

Outline of Final Research Achievements

In order to advance the cell therapy for cardiac arrhythmia, massive amount of iPS Cells-derived cardiomyocytes are necessary. Therefor the massive suspension culture system with a spinner flask and efficient purification strategy of cardiomyocytes were invented. Artificial nucleases were used to insert the fluorescent proteins into the specific locus of cardiac-specific transcription factor (NKX2-5) and conduction system specific gene. These cell lines were specifically differentiated and analyzed. Purified cardiomyocytes were transplanted into pigs and the transplantation strategy was evaluated. Cardiac telemetry transmitters were also implanted into pigs and the evaluation system of arrhythmia was established.

Free Research Field

循環器内科