2014 Fiscal Year Final Research Report
Development of biological dosimetry after the irradiation.
| Project/Area Number |
25550033
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Risk sciences of radiation and chemicals
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| Research Institution | Nagasaki University |
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Principal Investigator |
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| Research Collaborator |
HAYASHIDA Chisa
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Keywords | 放射線 / 変異 / 生物学的測定法 |
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| Outline of Final Research Achievements |
The aim of this research is to develop the biological dosimetry by measuring genome alterations after radiation exposure. Firstly, we developed the method for library construction from a tiny amount of DNA. DNA was amplified by using phi29 DNA polymerase with dUTP. After the amplification, 5’-end was converted to phosphate and 3’-end was to hydroxy group afer uracil DNA glycosylation and other enzymatic reactions. DNA library for sequencing was constructed from those DNA fragments for next generation sequencer. But we have not reached the step for measuring the DNA mutations after one-cell radiation exposure and for the development of biological dosimetry.
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| Free Research Field |
人類遺伝学
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