2015 Fiscal Year Final Research Report
A picture of whole DNA modification induced by carcinogens: Whole-genome analysis for sequence specificity and vulnerability
| Project/Area Number |
25550036
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Risk sciences of radiation and chemicals
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| Research Institution | Meijo University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
KOJIMA Nakao 鈴鹿医療科学大学, 医用工学部, 非常勤講師 (80333178)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Keywords | マウス胚性幹細胞 / 紫外線照射 / ゲノム修飾 / 未分化マーカー / 受精卵 / 脱メチル化 / LC/MS/MS |
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| Outline of Final Research Achievements |
The aim of this study is to take a whole picture of DNA damage induced by chemical carcinogens. I investigated benzo(a)pyrene which is containing in tobacco smoke and known as a potent carcinogen, and cyclobutane-pyrimidine dimer which is formed by UV exposure. In addition, this project achieved a great success in epigenetic analysis of mouse preimplantation zygote, in which methylcytidine was measured using a high-performance liquid chromatograph-triple quadrupole mass spectrometer.
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| Free Research Field |
化学物質影響評価
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