Development of fluorescent probes for monitoring of intracellular kinase activity to functional imaging of diseases

2015 Fiscal Year Final Research Report

• Project/Area Number: 25560202
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Biomedical engineering/Biomaterial science and engineering
• Research Institution: Kyushu University
• Principal Investigator: KATAYAMA YOSHIKI 九州大学, 工学(系)研究科(研究院), 教授 (70284528)
• Co-Investigator(Renkei-kenkyūsha): MORI Takeshi 九州大学, 大学院工学研究院, 准教授 (70335785)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 蛍光分子プローブ / プロテインキナーゼ / バイオ分析 / プロテインキナーゼ / 細胞内情報伝達 / 薬物探索

Outline of Final Research Achievements

Two types of molecular probing systems were developed for monitoring protein kinase activity. One is FRET type molecular system consisting of fluorescence-labeled cationic substrate peptide and anionic quantum dot and another one is polyion complex type nanoparticle consisting of TAMRA-labeled polyanion and dextran having cationic peptide substrate as side chain and small amount of Cy5 as internal standard. Both systems changed their fluorescence intensity with the phosphorylation on the peptide substrate due to decrease of the electrostatic interaction between peptide and QD or peptide conjugate dextran and polyanion. Also both systems could evaluate the IC50 of known some kinase inhibitors so the both systems could be useful for kinase inhibitors screening.

Free Research Field

分析化学、ケミカルバイオロジー、医用工学