Translocation of ROCK2 in endothelial cells after mechanical stimulation

2014 Fiscal Year Final Research Report

• Project/Area Number: 25560203
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Biomedical engineering/Biomaterial science and engineering
• Research Institution: Kyushu University
• Principal Investigator: KUDO Susumu 九州大学, 工学(系)研究科(研究院), 教授 (70306926)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: メカノトランスダクション / 内皮細胞 / DAG / ROCK2

Outline of Final Research Achievements

When endothelial cells are injured by mechanical stimulation using a microprobe, the endothelial cells migrate to the injured region. ROCK2 and Diacylglycerol (DAG) are involved in the regulation of endothelial cell migration after injury. In this study, we developed an experimental system to simultaneously visualize Ca2+ signaling and DAG distribution. Using this system, we mechanically stimulated a single cell to propagate Ca2+ waves and then examined DAG distribution. We investigated fluorescent images of intracellular Ca2+ levels and localization of DAG in response to mechanical injury. The fluorescent intensities decreased in the cells surrounding the stimulated cell, indicating the propagation of the Ca2+ wave. In the stimulated cell, there was a time-dependent increase in DAG in the periphery of the stimulated cell.

Free Research Field

バイオメカニクス