Technique for function analysis of membrane transport proteins using cell-derived liposomes

2015 Fiscal Year Final Research Report

• Project/Area Number: 25560204
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Biomedical engineering/Biomaterial science and engineering
• Research Institution: Kyushu Institute of Technology
• Principal Investigator: YASUDA Takashi 九州工業大学, 大学院生命体工学研究科, 教授 (80270883)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: リポソーム / 膜タンパク質 / トランスポータ / リンパ球細胞 / 微小孔 / マイクロ流路 / 蛍光共鳴エネルギー移動

Outline of Final Research Achievements

We developed a novel microdevice for transport protein analysis using a cell-sized liposome (extracellular vesicle) which was derived from a human lymphocytic cell with chemical stimulation. A liposome was immobilized on a microhole which was fabricated in a SiN (silicon nitride) diaphragm. Then, the liposome membrane was partially dissolved by surfactant treatment, which permitted the microhole to be covered with a portion of the membrane. The use of FRET (fluorescence resonance energy transfer) enabled detection of fluorescently-labeled glucose (2-NBDG) molecules which were transported by glucose transporter 1 (GLUT1) proteins present in a liposome membrane.

Free Research Field

バイオマイクロデバイス