Development of the sonochemical internalization method

2014 Fiscal Year Final Research Report

• Project/Area Number: 25560224
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Biomedical engineering/Biomaterial science and engineering
• Research Institution: Okayama University
• Principal Investigator: OHTSUKI Takashi 岡山大学, 自然科学研究科, 教授 (80321735)
• Co-Investigator(Kenkyū-buntansha): HARADA Atsushi 大阪府立大学, 大学院工学系研究科, 准教授 (50302774)
• Co-Investigator(Renkei-kenkyūsha): NAKATA Eiji 京都大学, エネルギー理工学研究所, 講師 (70467827)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: 超音波 / CPP / RNA / 音増感剤 / SCI

Outline of Final Research Achievements

We developed a method for cytosolic delivery of proteins and peptides using sonosensitizers and ultrasonic waves. This method is named as sonochemical internalization. Recently, many researchers have used cell-penetrating peptides (CPP) for cytosolic delivery of proteins or peptides. However, in many cases, the CPP-fused molecules are entrapped in endosomes. In this study, we attempted to use sonosensitizers and ultrasonic waves for the endosomal escape of the CPP-fused molecule. We confirmed the ultrasound-dependent endosomal escape slightly but certainly.

Free Research Field

生体関連化学