Construction of a cytotoxicity and function evaluation system using combinatorial cell clusters with magnetic nanoparticles

2015 Fiscal Year Final Research Report

• Project/Area Number: 25560248
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Medical engineering assessment
• Research Institution: National Center for Child Health and Development
• Principal Investigator: MIYAMOTO Yoshitaka 国立研究開発法人国立成育医療研究センター, 細胞医療研究部, リサーチアソシエイト (20425705)
• Co-Investigator(Kenkyū-buntansha): IKEUCHI Masashi 東京大学, 先端科学技術研究センター, 講師 (90377820)
• Co-Investigator(Renkei-kenkyūsha): NOGUCHI Hirofumi 琉球大学, 医学(系)研究科(研究院), 教授 (50378733) ; YAGI Tohru 東京工業大学, 大学院情報理工学研究科, 准教授 (90291096)
• Research Collaborator: HAYASHI Shuji ; SUZUKI Satoshi ; IKUTA Koji
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 細胞 / クラスター / 磁性ナノ粒子 / マイクロデバイス / 移植医療 / 細胞医療 / 毒性評価 / 医療技術

Outline of Final Research Achievements

This study aims to construct an evaluation system by combinatorial cell clusters. The cell clusters with various sizes were successfully prepared efficiently on our developed micro-fabricated device in a one-cell seeding. Furthermore, commercially available MRI contrast agents and our magnetic nanoparticles for MRI imaging were assessed for toxicity and cell functions by combinatorial hepatocyte clusters formed on this device. As a result, resultant clusters were also successfully evaluated for cell viability, cell proliferation, cytotoxicity, and functions at the protein level. Furthermore, it was possible to confirm the reproducibility and efficacy of other cell types such as adipose-derived stem cells on their toxicity and functions.

Free Research Field

複合領域、再生・移植医療、細胞・組織工学、医療技術評価