Development of an Active/Inactive Dual Probe Photoaffinity Labeling System for Identification of Natural Product Binding Proteins

2014 Fiscal Year Final Research Report

• Project/Area Number: 25560400
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Biomolecular chemistry
• Research Institution: Tokyo University of Agriculture and Technology
• Principal Investigator: SAKURAI Kaori 東京農工大学, 工学(系)研究科(研究院), 准教授 (50447512)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: フォトアフィニティープローブ / フォトアフィニティーラベリング / 天然物リガンド / OSW-1 / 結合タンパク質

Outline of Final Research Achievements

Identification of binding proteins is critical for understanding the mechanism of action for natural products. In this study, we synthesized an active photoaffinity probe based on an antitumor saponin OSW-1 and corresponding inactive photoaffinity probes to apply the previously developed dual-probe photoaffinity labeling method for the identification of OSW-1 binding proteins. We showed that the active and inactive photoaffinity probes we developed were useful in detecting candidate proteins selective toward the active probe.

Free Research Field

生物有機化学・ケミカルバイオロジー