2014 Fiscal Year Final Research Report
Development of in vitro vascular network model for angiogenesis research
| Project/Area Number |
25600057
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Nano/Microsystems
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| Research Institution | The University of Tokyo |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
SUEHIRO Junichi 杏林大学, 医学部, 助教 (80572601)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Keywords | 血管新生 / マイクロ流路デバイス / in vitroモデル / コラーゲン / 血管内皮細胞 |
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| Outline of Final Research Achievements |
Tissues in disease such as inflammation, angiogenesis and cancer metastasis, are highly complex, thus sophisticated model is required to understand these mechanism actions at cellular and tissue levels. In this study, we prepared collagen microchannels by using collagen gels and PDMS hosting chamber including a needle (120 micrometer in diameter) as a channel mold. By using the device, continuous lumen structure of human umbilical vascular endothelial cells (HUVECs) surrounded by collagen gels can be formed. This vasculature model allows continuous monitoring of the vascular sprouting responding to the angiogenic factors. Compared to the traditional 2D culture system, our model is very useful for the investigation of the vascular disease at both cellular and tissue levels.
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| Free Research Field |
組織工学
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