2014 Fiscal Year Final Research Report
In vivo imaging probes for visualization of activated osteoclasts
Project/Area Number |
25620133
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Bio-related chemistry
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Research Institution | Osaka University |
Principal Investigator |
KIKUCHI Kazuya 大阪大学, 工学(系)研究科(研究院), 教授 (70292951)
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Project Period (FY) |
2013-04-01 – 2015-03-31
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Keywords | pH感受性プローブ / 破骨細胞 / 二光子蛍光イメージング |
Outline of Final Research Achievements |
Osteoclasts are resorptive cells of bone and their activity is involved in the development of osteoporosis and rheumatoid arthritis. Elucidation of the mechanism of the bone resorption is highly important for the therapeutic applications. In this study, we developed a novel fluorescent probe “pHocas-3” that has low-pH sensitivity, high photostablity, and in vivo bone delivery efficiency. By administrating of this fluorescent probe in living mice, we achieved intravital fluorescence imaging of activated osteoclasts during a long time period using a two photon fluorescence microscopy. These results demonstrated that intravital fluorescence imaging using pHocas-3 will be a useful tool for investigating of the dynamics of activated osteoclasts in vivo.
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Free Research Field |
ケミカルバイオロジー
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