2014 Fiscal Year Final Research Report
Functional analysis of presynaptic gap junction by single terminal electroporation
Project/Area Number |
25640001
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Neurophysiology / General neuroscience
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Research Institution | Hokkaido University |
Principal Investigator |
KAMIYA Haruyuki 北海道大学, 医学(系)研究科(研究院), 教授 (10194979)
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Project Period (FY) |
2013-04-01 – 2015-03-31
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Keywords | 脳・神経 / 神経科学 / 生理学 / 薬理学 / シグナル伝達 |
Outline of Final Research Achievements |
In this study, we aimed at elucidating the roles of two modes of presynaptic gap junction, i.e. axo-axonal gap junction coupling and axo-dendritic gap junction coupling at the hippocampal mossy fiber synapses, which have been suggested to exsist on the basis of morphological analysis using connexin 36 immunoreactivity. Application of a gap junction blocker, meclofenamic acid, reversibly suppressed the excitatory postsynaptic potentials at the mossy fiber-CA3 synapse, supporting the possibility that the hippocampal mossy fiber synapse might be a unique mixed synapse composed of both chemical and electrical components of synaptic transmission.
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Free Research Field |
神経生理学
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