Voltage-dependent synapse formation by voltage dependent potassium ion channel, KCNX

2014 Fiscal Year Final Research Report

• Project/Area Number: 25640022
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Nerve anatomy/Neuropathology
• Research Institution: Hokkaido Bunkyo University
• Principal Investigator: KIMURA Kazushi 北海道文教大学, 人間科学部, 教授 (20314180)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: イオンチャネル / シナプス

Outline of Final Research Achievements

In this study, I have tried to elucidate the role of KCNX, which was identified as a new cell adhesion molecule, in synapse formation. To reveal the localization of KCNX in brain, I prepared GFP-KCNX knock-in mouse and stained the brain sections with anti-GFP antibody. Immunoreactivity for GFP were localized as dots in stratum lucidum of the CA3 area, where mossy fiber terminals form synapses with pyramidal cells. Next, to reveal function of KCNX, I performed immunoprecipitation of anti-KCNX antibody from mouse brain membrane extracts. CLIP115, one of plus-end tracking proteins and Dynamin, a GTPase responsible for endocytosis were co-immunoprecipitaed with KCNX. These result suggest that KCNX is involved in regulating microtubules and endocytosis in neuron.

Free Research Field

神経細胞生物学