2015 Fiscal Year Final Research Report
A study of neuronal activity-dependent transcription factor dynamics using single-molecule imaging
Project/Area Number |
25640035
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Neurochemistry/Neuropharmacology
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Research Institution | Osaka University |
Principal Investigator |
SUGO Noriyuki 大阪大学, 生命機能研究科, 助教 (20372625)
|
Project Period (FY) |
2013-04-01 – 2016-03-31
|
Keywords | 神経細胞 / 神経活動依存的 / 転写 / 1分子イメージング |
Outline of Final Research Achievements |
Neuronal activity-dependent gene expression is necessary for circuit formation and plasticity, although it remains unclear how neuronal activity affects transcription mechanisms. Here, we studied dynamics of transcription factor in cortical neurons, using a single-molecule imaging technique with a highly inclined and laminated optical sheet microscope. We found that the transcription factor CREB appeared repeatedly at highly restricted nuclear locations in response to neuronal activity.
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Free Research Field |
神経科学
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