Production of monozygous Japanese monkeys as animal models for Cognitive Neuroscience.

2014 Fiscal Year Final Research Report

• Project/Area Number: 25640045
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Laboratory animal science
• Research Institution: Kyoto University
• Principal Investigator: OKAMOTO Munehiro 京都大学, 霊長類研究所, 教授 (70177096)
• Co-Investigator(Kenkyū-buntansha): SOTOMARU Yusuke 広島大学, 自然科学研究支援開発センター, 教授 (90309352)
• Co-Investigator(Renkei-kenkyūsha): NOBUKIYO Asako 広島大学, 自然科学研究支援開発センター, 助教 (10294563) ; HATAKEYAMA Teruhiko 広島大学, 自然科学研究支援開発センター, 技術員 (50452595) ; ISA Tadashi 生理学研究所, 生命化学研究科, 教授 (20212805)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: 一卵性多子 / ニホンザル / 高次脳機能

Outline of Final Research Achievements

In order to produce monozygous Japanese monkeys as animal models for Cognitive Neuroscience, we examined and improved associated embryological engineering techniques. ① It was revealed that it was effective to make the dose of the hormone half of the known report to raise efficiency of the egg collection. ② TEST Yolk Buffer was suitable for the cryopreservation of the sperm. After melting, sperm was treated by the BO solution with 1mM Caffeine and 1mM dbcAMP. Such sperm could use n‐vitro fertilization. ③ Although an egg fertilized in vitro or splitter fertilized eggs were transplanted to female Japanese macaques, we were not able to confirm the pregnancy.

Free Research Field

実験動物学