Functional analysis of chromosome specific clustered trap region (CSCT).

2015 Fiscal Year Final Research Report

• Project/Area Number: 25640052
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Laboratory animal science
• Research Institution: Kumamoto University
• Principal Investigator: ARAKI MASATAKE 熊本大学, 生命資源研究・支援センター, 准教授 (80271609)
• Co-Investigator(Kenkyū-buntansha): ARAKI Kimi 熊本大学, 生命資源研究・支援センター, 教授 (90211705)
• Research Collaborator: YOSHINOBU Kumiko 熊本大学, 生命資源研究・支援センター, 助教 (20274730)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 遺伝子トラップ / CSCT / EGTC / ゲノム編集 / 染色体相同組換え / 減数分裂

Outline of Final Research Achievements

We found new genome element, CSCT (Chromosome Specific Clustered Trap region).
Using CRISPR/Cas9 system, CSCT13 region (1.6 Mbp) was deleted in the mouse ES cells (CSCT13 KO). We could establish CSCT13 KO mouse line. Mating between homozygotes gave relatively small number of pups. Moreover, CSCT13 KO mice showed comparatively low rate of homologous recombination during meiosis for the region corresponding to CSCT13. On the other hand, outside of this region showed up-regulation of homologous recombination during meiosis.
This study suggest that CSCT13 might be related with the early embryogenesis and homologous recombination during meiosis.

Free Research Field

実験動物学