Identification of long non-coding RNA associated protein complexes using mouse artificial chromosome

2014 Fiscal Year Final Research Report

• Project/Area Number: 25640114
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: System genome science
• Research Institution: Tottori University
• Principal Investigator: KUGOH HIROYUKI 鳥取大学, 医学(系)研究科(研究院), 教授 (40225131)
• Research Collaborator: 大平 嵩人 ; 砂村 直洋 ; 稲岡 大悟
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: long noncoding RNA / Xist / LIT1/KCNQ1OT1 / chromosome / MAC

Outline of Final Research Achievements

In this study, we established NIH/3T3 and HeLa cells containing MAC and human chromosome 21 that express Xist RNA by a doxycycline (Dox). Xist RNA territories were observed both on MAC and human chromosome 21 by Dox-dependent Xist expression. However, genes that contain selectable marker located on MAC were expressed despite on cis-accumulation of Xist RNA on MAC. On the other hand, it was observed accumulation of H3K27me3 on human chromosome 21 expressing Xist RNA. These results suggest that the establishment of XCI by Xist RNA may play an important role in a special chromatin region as elements for cis-silencing on chromosome.In addition, we developed tool for recovery of human chromosome 21 expressing Xist RNA using FLAG/dCas9-EGFP.

Free Research Field

染色体工学