Novel development and applications of the reagents for chemical scission of targetted proteins as chemical scissors

2014 Fiscal Year Final Research Report

• Project/Area Number: 25650038
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Functional biochemistry
• Research Institution: Kinki University
• Principal Investigator: NOZAKI Osamu 近畿大学, 医学部附属病院, 講師 (50164687)
• Co-Investigator(Kenkyū-buntansha): SHIZUMA Motohiro 地方独立行政法人・大阪市立工業研究所, 生物・生活材料研究部, 研究員 (40416318)
• Research Collaborator: KAWAMOTO Hiroko
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: 蛋白切除 / 蛋白過酸化イソインドール / 化学発光 / 蛍光画像測定 / 質量解析 / 試薬開発 / 血清蛋白分画 / o-phthalaldehyde

Outline of Final Research Achievements

Development of the novel reagent for chemical scission of targeted proteins and its clinical application　were studied. The reagents those demolish proteins under mild chemical conditions have been requested for treatment of patients and research of diseases. The reagents were protein peroxyisoindoles of proteins, and were prepared by the reaction with hydrogen peroxide following to the reaction with o-phthalaldehyde and thiol compounds. The production of protein peroxyisoindole were confirmed by fluorescence imaging. The peroxyisoindole was oxidized to emit light, accompanying of disappearance of the fluorescence image. This meant demolition of proteins. The reagent of peroxyisoindole was also applied to serum protein fractionated by electrophoresis resulting in successful cleavage. In conclusion, the novel reagents of proteins allowed simultaneous protein scission and monitoring of the accompanying chemiluminescence.

Free Research Field

臨床検査医学、内科学、分析化学