Development a cell selection method and analysis of cell-cell communication using a microfabrication technique

2015 Fiscal Year Final Research Report

• Project/Area Number: 25650058
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Biophysics
• Research Institution: Tokyo Medical and Dental University
• Principal Investigator: Terazono Hideyuki 東京医科歯科大学, 生体材料工学研究所, 助教 (30398143)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 神経ネットワーク / 人工神経細胞回路 / マイクロファブリケーション技術

Outline of Final Research Achievements

Three techniques, a microprocessing-assisted cell-systematic evolution of ligands by exponential enrichment method, a single cell collection method by measuring firing rate of neuron and a micro-patterning using an agarose microfabrication technique, were developed to clarify the fundamental mechanism of the neuronal network. As the results, single strand DNA aptamers that bind cell surfaces was able to be collected by a modified aptamer selection method. Moreover, I clarified a network structure-dependent firing pattern by constructing several neuronal networks using single cell collection method and the microfabrication technique.

Free Research Field

生物物理