co-localizatonome for finding new protein-protein interaction

2014 Fiscal Year Final Research Report

• Project/Area Number: 25650068
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Cell biology
• Research Institution: Nara Institute of Science and Technology
• Principal Investigator: KIMATA Yukio 奈良先端科学技術大学院大学, バイオサイエンス研究科, 准教授 (60263448)
• Co-Investigator(Renkei-kenkyūsha): KOHNO Kenji 奈良先端科学技術大学院大学, バイオサイエンス研究科, 教授 (50142005)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: タンパク質 / 蛍光顕微鏡 / 酵母

Outline of Final Research Achievements

In eukaryotic cells, many proteins show uneven distribution. Some proteins are located on specific cellular compartments. Some proteins shows more unique distribution, which is sometimes hard to be explained. If two proteins show overlapping distributions, it may suggest that they form protein complex and /or work together. Such a knowledge may lead to finding of a new cellular complex. To this end, I have developed methodologies toward co-localizationome, which is the genome-wide and comprehensive dataset of protein co-localization. One protein is labelled by the green fluorescent protein, and another protein is labelled by the red fluorescent protein. Then Saccharomyces cerevisiae cells expression two fluorescently labelled proteins are systematically observed under a fluorescent microscope.

Free Research Field

細胞生物学