Picobiological analysis of the ER and Golgi stress responses

2014 Fiscal Year Final Research Report

• Project/Area Number: 25650070
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Cell biology
• Research Institution: University of Hyogo
• Principal Investigator: YOSHIDA Hiderou 兵庫県立大学, 生命理学研究科, 教授 (60378528)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: 小胞体 / ゴルジ体 / ストレス応答 / 構造生物学 / 結晶構造解析 / 転写 / スプライシング / シャペロン

Outline of Final Research Achievements

Secretory proteins are synthesized and folded with endoplasmic reticulum (ER) chaperones in the ER, and received various post-translational modifications such as glycosylation. The ER and Golgi stress responses are the mechanisms that augment function of each organelle in accordance with cellular demands. In this project, we planned to reveal the mammalian molecular mechanisms of the ER and Golgi stress responses through the structural analyses of pATF6(P), pXBP1(U) and TFE3, key regulators of the ER and Golgi stress response. Finaly, we obtained pXBP1(U) protein for the analysis of X-ray crystallography.

Free Research Field

細胞生物学