Search for novel trafficking pathway dependent on microtubule plus-end-tracking proteins (+TIPs)

2014 Fiscal Year Final Research Report

• Project/Area Number: 25650071
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Cell biology
• Research Institution: The Institute of Physical and Chemical Research
• Principal Investigator: KIYOSUE Yuko 独立行政法人理化学研究所, ライフサイエンス技術基盤研究センター, ユニットリーダー (90568403)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: 細胞骨格 / 微小管 / 細胞極性 / 物質輸送 / 細胞分裂 / 高解像イメージング

Outline of Final Research Achievements

This research project aimed at search for novel trafficking pathway dependent on microtubule plus-end-tracking proteins (+TIPs), which connect microtubule ends with subcellular structures, on the supposition that these molecules serve as platforms for loading/unloading or sorting of cargos transported by motor proteins. Our analyses focused on LL5 protein and APC tumor suppressor protein led to the discovery of novel regulatory mechanisms for trafficking and mouse phenotypes. We also found a crosstalk between APC tumor suppressor and an important mitotic kinase involved in the transport of chromosomes. In addition, we succeeded in the 3D tracking of microtubule growth throughout the cell cytoplasm using the recently developed lattice light-sheet microscope, which enables 3D scanning at subsecond intervals with high spatial resolution.

Free Research Field

細胞生物学