Optogenetic labeling of neural circuits for gene expression analysis in vivo

2014 Fiscal Year Final Research Report

• Project/Area Number: 25650074
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Developmental biology
• Research Institution: The University of Tokyo
• Principal Investigator: CHIHARA Takahiro 東京大学, 薬学研究科(研究院), 准教授 (00431891)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: 遺伝子発現 / 神経細胞 / マイクロアレイ / ショウジョウバエ

Outline of Final Research Achievements

In order to understand the development and function of neural circuits, it is crucial to know which gene is expressed in each neuron of the brain. In this study, we aimed to develop the efficient method to analyze the gene expression profile of neurons of interest in the Drosophila brain. As a result, we succeeded to establish the method to perform microarray analysis using total RNA isolated from genetically labeled neurons in the developing Drosophila brain.

Free Research Field

神経発生遺伝学