2013 Fiscal Year Final Research Report
identification of regulatory factors for generation of neurons by using live imaging of embryos
| Project/Area Number |
25650076
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Developmental biology
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| Research Institution | Kyoto University |
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Principal Investigator |
SEHARA Atsuko 京都大学, 再生医科学研究所, 教授 (60209038)
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| Project Period (FY) |
2013-04-01 – 2014-03-31
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| Keywords | 細胞分化 / 増殖因子 / 神経分化 / ゼブラフィッシュ / ライブイメージング / ニューレグリン |
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| Research Abstract |
During brain development, neural progenitor cells (NPCs) proliferate and produce post-mitotic neurons. The extent of NPC expansion and the timing of neuron production should impact on the size and shape of the brain. Here, we show that neuronal generation from NPCs is not a stochastic event, but instead a spatially and temporally regulated process and depends on Neuregulin 1 type II (NRG1-II), an isoform of an EGF-related ligand NRG1, using the optic tectum (OT) of zebrafish embryos as a model system. Knocking-down NRG1-II or treatment with AG1478, an ErbB inhibitor, impaired neuron production from sub-basal NPCs prominently. The removal of AG1478 resumed sub-basal mitoses without affecting mitoses in the apical ventricular (V) region. Injection of soluble human NRG1 into the brain ameliorated neurogenesis of NRG1-II-depleted embryos, implying involvements of NRG1-ErbB signaling in neuron production in the developing vertebrate brain.
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Research Products
(9 results)
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[Journal Article] Fetal Skeletal Muscle Progenitors Have Regenerative Capacity After Intramuscular Engraftment in Dystrophin Deficient Mice2013
Author(s)
Sakai, H., Sato, T., Sakurai, H., Yamamoto, T., Hanaoka K., Montarras, D., Sehara-Fujisawa, A
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Journal Title
PLoS ONE
Volume: 8(5)
Pages: e63016
DOI
Peer Reviewed
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[Journal Article] Efficient and reproducible myogenic differentiation from human iPS cells : prospects for modeling Miyoshi Myopathy in vitro2013
Author(s)
Tanaka, A., Woltjen, K., Miyake, K., Hotta, A., Ikeya, M., Yamamoto, T., Nishino, T., Shoji, E., Sehara-Fujisawa, A., Manabe, Y., Fujii, N., Hanaoka, K., Era, T., Yamashita, S., Isobe, K., Kimura, E., and Sakurai, H
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Journal Title
PLoS ONE
Volume: 8(4)
Pages: e61540
DOI
Peer Reviewed
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[Presentation] Roles of ADAM Proteases in Development of Zebrafish2013
Author(s)
Fuminori Sato, Hiroyuki Kangawa, Hiroyuki Arai, Kazuya Tsumagari, Atsuo Kawahara, Koichi Kawakami, Atsuko Sehara-Fujisawa
Organizer
the 8th General Meeting of the International Proteolysis Society
Place of Presentation
Cape Town, South Africa
Year and Date
2013-10-23
Invited
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