Evolutional study on the membrane co-transport system in plant cell.

2014 Fiscal Year Final Research Report

• Project/Area Number: 25650128
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Evolutionary biology
• Research Institution: Kobe University
• Principal Investigator: MIMURA Tetsuro 神戸大学, 理学(系)研究科(研究院), 教授 (20174120)
• Research Collaborator: FUJIWARA Hitomi 神戸大学 ; OHNISHI Miwa 神戸大学 ; ANEGAWA Aya 神戸大学 ; SAKAYAMA Hidetoshi 神戸大学 ; FUKAKI Hidehiro 神戸大学 ; ISHIZAKI Kimitsune 神戸大学 ; SHICHIJO Chizuko 神戸大学 ; KOSUGE Keiko 神戸大学 ; TOYOKURA Koichi 神戸大学 ; GOH Tatsuaki 神戸大学 ; NISHIYAMA Tomoaki 金沢大学 ; SEKIMOTO Hiroyuki 日本女子大学
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: 共役輸送 / リン酸 / シャジクモ / ゼニゴケ / 進化

Outline of Final Research Achievements

The membrane co-transport systems work on the uptake of nutrition or extrusion of wastes. This is the fundamental mechanism of all living cells. There are two kinds of co-transport systems; H+ coupled or Na+ coupled. The land plants usually have H+ coupled co-transport systems. We have reported that the phosphate (Pi) transport system of characean plant, which is an ancestor of land plants is a Na+ coupled type. In the present study we succeeded in sequencing the full length of a gene of the Pi transporter in Chara cells. From sequence analysis, Chara Pi transporter was the ancestor type of all land plants and formed a single clade together with Spirogyra. In Chara Pi transporter gene, amino acid residue required for H+ coupled transport was not kept, and this strongly suggests that Chara Pi transporter is a Na+ coupled type. In addition, we are analyzing about Pi transport systems of the first land plant; the liverwort.

Free Research Field

植物細胞生物学