2014 Fiscal Year Final Research Report
Bacterial autecology in soil environments by using proteomics
| Project/Area Number |
25650139
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Ecology/Environment
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| Research Institution | Hokkaido University |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Keywords | メタプロテオーム / 土壌細菌 / 個生態学 |
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| Outline of Final Research Achievements |
To detect soil-specific expressed genes, comparative proteome analysis of the global behavior of Pseudomonas putida F1 between three soils and liquid batch culture was performed. (1) We studied direct and indirect protein extraction methods by comparing the number of the identified proteins and their functional and taxonomical distributions. We demonstrated the advantages and disadvantages of the two extraction methods that can be applied to complex natural soils. (2) Two genes and 1 operon consisted of 5 genes as a soil-specific expressed gene were identified. From expression factor analysis, one of the genes was expressed by NO. Expression of the operon was found to be the lack of certain soil components. It became possible to know the microenvironment surrounding the bacteria in this study.
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| Free Research Field |
ゲノム微生物学、微生物生態学
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