Transgenesis of insect-transmissible plant-pathogenic microorganisms

2014 Fiscal Year Final Research Report

• Project/Area Number: 25660034
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Plant protection science
• Research Institution: The University of Tokyo
• Principal Investigator: NAMBA Shigetou 東京大学, 農学生命科学研究科, 教授 (50189221)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: プラスミド / トランスジェネシス

Outline of Final Research Achievements

Due to difficulties in culturing insect-transmissible plant-pathogenic bacteria (such as phytoplasmas) in vitro and in manipulating them genetically, these organisms are one of the most poorly characterized groups of plant pathogens. In this study, we investigated a “transgenesis method” that aims genetic manipulation without culturing using phytoplasmas as model organisms. We specified the minimum unit of a phytoplasmal plasmid for a development of shuttle vector. We also searched a phytoplasmal promoter that is active in both plant and insect hosts. Using these results, we constructed a shuttle vector and a gene expression cassette.

Free Research Field

農学