2014 Fiscal Year Final Research Report
Analysis of salicylic acid receptor produced by novel in vitro translation system
| Project/Area Number |
25660037
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Plant protection science
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| Research Institution | Nagoya University (2014)
Kagawa University (2013) |
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Principal Investigator |
TADA Yasuomi 名古屋大学, 学内共同利用施設等, 教授 (40552740)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
|
| Keywords | 無細胞タンパク質合成系 |
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| Outline of Final Research Achievements |
Optimization of in vitro protein synthesis system was performed. We determined the optimum concentrations of amino acids, ATP and GTP used for the wheat germ-derived translation solution. Furthermore, we shortened the 3'UTR sequence attached to an ORF to stabilize in vitro transcribed mRNA. We achieved about 10-fold efficiency in producing any proteins with the above conditions.
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| Free Research Field |
植物生理学
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