Reverse toxicological search for new miticides from species specific target sites.

2014 Fiscal Year Final Research Report

• Project/Area Number: 25660044
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Plant protection science
• Research Institution: Kobe University
• Principal Investigator: TAKEDA Makio 神戸大学, (連合)農学研究科(研究院), 教授 (20171647)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: GABA受容体 / 殺ダニ剤 / 神経伝達物質 / 塩化物イオンチャンネル / ナミハダニ / RDL受容体 / ディルドリン抵抗性 / フィプロニル

Outline of Final Research Achievements

Cloning of GABA receptor from the two spotted spider mite (TSSM), Tetranychus urticae revealed a substitution of a critical amino acid at the second transmembrane damain of a GABA recptor, RDL that is conserved in insects. TSSM GABA receptor has a unique spectrum in action of mitocids and non-competitive GABAR agonists from ticks and insects. We made a chimera construct to narrow down the critical structure to establish effective screening for specific miticides, employing two electrode voltage clamp method. The result however indicated that not only the second trans-membarne domain but multiple other sites are sensitive to these compounds, especially Cyc-loop region, which complicated the strategy of effective screen system for specific miticidal compounds contrary to our original expectation.

Free Research Field

昆虫生理生化学・分子生物学