2014 Fiscal Year Final Research Report
Lysine biosynthetic pathway in a Streptomyces strain producing polylysine
| Project/Area Number |
25660064
|
|---|
| Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Applied microbiology
|
|---|
| Research Institution | Fukui Prefectural University |
|---|
Principal Investigator |
|
|---|
| Research Collaborator |
TAMAI Yuki
|
|---|
| Project Period (FY) |
2013-04-01 – 2015-03-31
|
| Keywords | 生合成 |
|---|
| Outline of Final Research Achievements |
ε-Poly-L-lysine (ε-PL), consisting of 25-35 L-lysine residues, is produced by Streptomyces albulus NBRC14147. Since ε-PL is produced as a secondary metabolite, high transcript levels of the ε-PL synthetic gene are observed during late-growth phase. Interestingly, however, the transcript level of the aspartokinase gene (ask gene) was low in the late-growth phase, although aspartokinase is a rate-limiting enzyme in the L-lysine biosynthetic pathway (DAP pathway). A draft-genome sequencing of S. albulus NBRC14147 revealed that this strain has isozyme genes involved in the DAP pathway. In addition, RT-PCR analysis demonstrated the growth-phase dependent operation of the isozyme genes.
|
| Free Research Field |
応用微生物学
|
