Screening of microorganisms producing a novel proteinase, hydrolysing D-peptide substrate

2014 Fiscal Year Final Research Report

• Project/Area Number: 25660067
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Applied microbiology
• Research Institution: Sojo University
• Principal Investigator: SHIN Takashi 崇城大学, 生物生命学部, 教授 (50179066)
• Co-Investigator(Kenkyū-buntansha): EKINO Keisuke 崇城大学, 生物生命学部, 准教授 (30310030) ; NOMURA Yoshiyuki 崇城大学, 生物生命学部, 教授 (40133566) ; DOBASHI Kazuyuki 崇城大学, 工学部, 教授 (90587833)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: D-ペプチド / プロテアーゼ

Outline of Final Research Achievements

Screening for a novel proteinase of microbial origin, D-peptide specific enzyme, was carried out. For simple procedure of detection of proteinase, short chain and/or long chain (30 amino residures) peptides, comprising with D-amino acid, were newly synthesized by solid-phase method or convinient procedures. Employing these subustrates enable the isolation of D-aspartic acid specific enzyme in the culture filtrate of a newly isolated actinomyces strain. Preliminary investigation of crude enzyme preparation, may prove the desirable enzyme characteristics. Another procedure for isolation of microorganism, assimilatig D-amino acid for growth, was carried out, to establish a specific microorganism culture collection.

Free Research Field

応用微生物学