Development of clonal propagation technology for nematode-resistant pines via somatic embryogenesis

2015 Fiscal Year Final Research Report

• Project/Area Number: 25660126
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Forest science
• Research Institution: Forestry and Forest Products Research Institute
• Principal Investigator: Maruyama Tsuyoshi 国立研究開発法人 森林総合研究所, 生物工学研究領域, 領域長 (20353865)
• Co-Investigator(Kenkyū-buntansha): HOSOI Yoshihisa 国立研究開発法人 森林総合研究所, 生物工学研究領域, チーム長 (50353842)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 組織培養 / クローン増殖 / 栄養組織外植体 / カルス / 多芽体 / 器官分化 / プロトプラスト培養 / ザイセンチュウ抵抗性マツ

Outline of Final Research Achievements

The study aimed to develop basic techniques for the propagation of adult nematode-resistant pine trees from vegetative material using tissue culture techniques. The culture conditions for the induction of somatic embryogenic cells, shoot primordia and adventitious buds, and for the protoplast culture were investigated. It was possible to perform efficiently callus induction from shoot-tip and needle sections of adult Japanese pines. Also, it was confirmed that by using the shoot-tip sections of Japanese black pine, are possible induction and growth of adventitious buds. Further, the appropriate culture conditions for the elongation and rooting of shoots were examined.

Free Research Field

農学