2014 Fiscal Year Final Research Report
Studies on new mechanisms of oxytocin on sperm and its application to reproductive technologies in mammals
| Project/Area Number |
25660215
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Animal production science
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| Research Institution | Azabu University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
ITO Junya 麻布大学, 獣医学部, 准教授 (30454143)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Keywords | 精子 / 凍結保存 / オキシトシン / オキシトシンレセプター / 運動精子率 / 凍結融解精子 / ラット / ブタ |
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| Outline of Final Research Achievements |
In general, motility of cryopreserved sperm is low, and that its fertility is low, too. The aim of this study was to develop a method to efficiently restore offspring from cryopreserved sperm, we examined that effect of oxytocin (OXT) on motility of frozen/thawed sperm. In addition, we examined existence of oxytocin receptors (OXTR) in sperm. At 5 h culture after thawing, the motility of sperm exposed to 10 nM of the Peptide OXT was significantly higher than the control (0 nM). In the porcine sperm, there is no effect of OXT on motility of the frozen/thawed sperm. In the present study, OXTR were detected in frozen/thawed sperm in the rat, mouse, and pig. The results of the present study showed that motility of cryopreserved sperm could be improved by exposure to OXT.
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| Free Research Field |
動物生殖工学
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