2015 Fiscal Year Final Research Report
Neutralizing epitope(s) recognized by the pigs endured PRRS through phage display
| Project/Area Number |
25660226
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Veterinary medical science
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| Research Institution | Kyoto University |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Keywords | ウイルス / ブタ / ワクチン / 抗体 / ファージディスプレイ / 中和 / ライブラリー |
|---|
| Outline of Final Research Achievements |
The cDNA was synthesized from the hilar lymph node cells of PRRSV infected and protected pig. The molecules of DNA fragment coding for the variable regions of antibody was ligated into the linear molecules (scFv) to prepare a library into a phagemid vector. In addition, the virus particles to construct a system of ELISA to the antigen. From the library (about one million clones) three attempts to screening by panning, but it was not possible to select an antibody molecule that binds to a PRRSV antigen.
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| Free Research Field |
ウイルス学
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