Research for molecular basis of epigenetic operation in transcription by inducing nuclear actin filaments

2015 Fiscal Year Final Research Report

• Project/Area Number: 25660286
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Applied molecular and cellular biology
• Research Institution: Tohoku University
• Principal Investigator: Harata Masahiko 東北大学, (連合)農学研究科(研究院), 准教授 (70218642)
• Co-Investigator(Renkei-kenkyūsha): MITANI Tasuku 近畿大学, 先端技術総合研究所, 教授 (10322265)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 細胞核 / アクチン / 転写 / エピジェネティック

Outline of Final Research Achievements

We induced the formation of nuclear actin filaments in cultured cells, and anlyzed gene transcription. In the cells, the expression of multiple genes, including OCT4, were changed. We then analyzed the effect of nuclear actin filaments on Wnt/beta-catenin signaling. We found that nuclear actin filaments induced the accumulation of beta-catenin in the nucleus and activate targets genes. In addition, nuclear actin filaments associated the promoter of the genes.

Free Research Field

分子細胞生物学