Development of a novel protein production system in mammalian cells.

2014 Fiscal Year Final Research Report

• Project/Area Number: 25660295
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Applied molecular and cellular biology
• Research Institution: Kyoto University
• Principal Investigator: MASUDA Seiji 京都大学, 生命科学研究科, 准教授 (20260614)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: mRNA / タンパク質 / 輸送受容体

Outline of Final Research Achievements

The proteins for the clinical use are produced in mammalian cells because they needs post translational modification such as glycosylation, formation of adequate disulfide-bond, acylation and phosphorylation for their activity in human body. The protein production in mammalian cells, however, takes much cost than that in bacteria. The author found that the mRNA export process becomes the limited step than transcription or translation process. In the present study, mRNA export receptor is created to establish a novel protein production system in mammalian cells by modifying the sequence of mRNA export receptor. The mutant receptor produce more reporter protein than the wild type export receptor.

Free Research Field

分子細胞生物学